Is minimal: No
| Compound | Amount (mM) | 
|---|---|
| ACES | 54.9 | 
| Biotin | 0.000409 | 
| Calcium chloride anhydrous | 0.00374 | 
| Cobalt chloride | 0.00202 | 
| Cupric sulfate | 0.0001 | 
| Ferrous sulfate | 0.367 | 
| Glycerol | 27.4 | 
| Glycine | 1.33 | 
| L-Alanine | 1.12 | 
| L-Arginine | 0.574 | 
| L-Asparagine | 15.1 | 
| L-Aspartate | 0.751 | 
| L-Glutamate | 0.68 | 
| L-Isoleucine | 0.762 | 
| L-Leucine | 0.762 | 
| L-Phenylalanine | 0.605 | 
| L-Serine | 0.952 | 
| Magnesium sulfate | 0.8869 | 
| Manganese chloride | 0.000101 | 
| Potassium dihydrogen phosphate | 1.63 | 
| Potassium hydroxide | 1.0 | 
| Pyruvate | 9.09 | 
| Sodium bicarbonate | 0.5 | 
| Tween 80 | 1.53 | 
| Zinc sulfate | 0.00141 |